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ADVANCED MASS SPECTROMETRY
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ADVANCED MASS SPECTROMETRY
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Academic year 2020/2021
- Course ID
- BIO0212
- Teacher
- Dott.ssa Federica Dal Bello
- Degree course
- [0101M21] Molecular Biotechnology
- Year
- 2nd year
- Teaching period
- To be defined
- Type
- Elective
- Credits/Recognition
- 3
- Course disciplinary sector (SSD)
- CHIM/01 - chimica analitica
- Delivery
- Formal authority
- Language
- English
- Attendance
- Obligatory
- Type of examination
- Written
- Prerequisites
- Elements of analytical chemistry, organic chemistry and biochemistry.
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Sommario del corso
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Course objectives
Acquisition of competences in the field of mass spectrometry analyses.
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Results of learning outcomes
Analytical methods study and development skills
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Course delivery
The course is delivered face-to-face, in class lectures and lab visits. The total course time is 24 hours.
After the recent emergency situation, the teaching will be made compatible with an integrated learning environment in e-learning mode, suitable for face-to-face situations, those in which teaching is provided entirely online and forms of blended teaching.
Depending on the changing situations, the platform course will welcome: lesson materials (video recordings and / or audio presentations); exercises to be carried out online, but also face-to-face; in-depth teaching materials that can be discussed through a forum and / or in the classroom during the lesson-debate. The objective of the learning environment is to create continuity between the different teaching moments and between the different situations (remote activities and face-to-face activities).
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Learning assessment methods
Written evaluation of a scientific paper. Depending on the recent emergency situation, the exam may be in oral remote (webex) mode.
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Support activities
Laboratory practical activity on a high resolution MS system
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Program
LC-MS and GC-MS applications. Advanced applications and mrthodologies in biology and biotechnologies.
Metabolomics by Mass Spectrometry. Targeted and Untargeted metabolic profiling. Multivariate analysis, PCA, OPLS-DA, HCA, Pathway Analysis. Online/Offline Database Searching, Structural Identification by MS/MS Analysis. High-Definition Mass Spectrometry, Ion Mobility Mass
Spectrometry. MALDI-MSI, DESI-MSI, PESI-MS. Technique of Cryosectioning, Lazar Microdissection. Stability of Metabolites in Biological Samples. Application of Mass Spectrometry for Clinical Studies, and a large-scale cohort study. Multi-Omics Analysis.Suggested readings and bibliography
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Mass Spectrometry Reviews
Journal of the American Society of Mass Spectrometry
Journal of Mass Spectrometry
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Note
The teaching activity may vary according to the limitations imposed by the current health crisis. In any case, the remote mode is ensured throughout the academic year
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