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ADVANCED MASS SPECTROMETRY

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ADVANCED MASS SPECTROMETRY

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Academic year 2020/2021

Course ID
BIO0212
Teacher
Dott.ssa Federica Dal Bello
Degree course
[0101M21] Molecular Biotechnology
Year
2nd year
Teaching period
To be defined
Type
Elective
Credits/Recognition
3
Course disciplinary sector (SSD)
CHIM/01 - chimica analitica
Delivery
Formal authority
Language
English
Attendance
Obligatory
Type of examination
Written
Prerequisites
Elements of analytical chemistry, organic chemistry and biochemistry.
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Sommario del corso

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Course objectives

Acquisition of competences in the field of mass spectrometry analyses.

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Results of learning outcomes

Analytical methods study and development skills

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Course delivery

The course is delivered face-to-face, in class lectures and lab visits. The total course time is 24 hours.

After the recent emergency situation, the teaching will be made compatible with an integrated learning environment in e-learning mode, suitable for face-to-face situations, those in which teaching is provided entirely online and forms of blended teaching.

Depending on the changing situations, the platform course will welcome: lesson materials (video recordings and / or audio presentations); exercises to be carried out online, but also face-to-face; in-depth teaching materials that can be discussed through a forum and / or in the classroom during the lesson-debate. The objective of the learning environment is to create continuity between the different teaching moments and between the different situations (remote activities and face-to-face activities).

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Learning assessment methods

Written evaluation of a scientific paper. Depending on the recent emergency situation, the exam may be in oral remote (webex) mode.

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Support activities

Laboratory practical activity on a high resolution MS system

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Program

LC-MS and GC-MS applications. Advanced applications and mrthodologies in biology and biotechnologies. 

Metabolomics by Mass Spectrometry. Targeted and Untargeted metabolic profiling. Multivariate analysis, PCA, OPLS-DA, HCA, Pathway Analysis. Online/Offline Database Searching, Structural Identification by MS/MS Analysis. High-Definition Mass Spectrometry, Ion Mobility Mass
Spectrometry. MALDI-MSI, DESI-MSI, PESI-MS. Technique of Cryosectioning, Lazar Microdissection. Stability of Metabolites in Biological Samples. Application of Mass Spectrometry for Clinical Studies, and a large-scale cohort study. Multi-Omics Analysis.

Suggested readings and bibliography

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Mass Spectrometry Reviews

Journal of the American Society of Mass Spectrometry

Journal of Mass Spectrometry



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Note

The teaching activity may vary according to the limitations imposed by the current health crisis. In any case, the remote mode is ensured throughout the academic year

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Last update: 28/09/2020 15:40
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