Master Program in Molecular Biotechnology

ADVANCED MASS SPECTROMETRY

ADVANCED MASS SPECTROMETRY

Academic year 2024/2025

• ADVANCED MASS SPECTROMETRY (BIO0217)
  Biotechnological and Chemical Sciences in Diagnostics

Sommario del corso

• Course objectives
• Results of learning outcomes
• Program
• Course delivery
• Learning assessment methods
• Support activities
• Suggested readings and bibliography
• Teaching tools
• Course card

Course objectives

Acquisition of competences in the field of mass spectrometry analyses.

The objective of the course is to provide students with the principles for the development and application of methods based on (high resolution) mass spectrometry in the biochemical and biopharmaceutical fields.

Results of learning outcomes

Analytical methods study and development skills

At the end of the course, students will be able to:

• Understand the value of mass spectrometry techniques to address biochemical and biotechnological problems
• Know when and how applying specific mass spectrometry techniques
• Analyze the obtained results (autonomy of judgment)

Program

LC-MS and LC-HRMS applications. Advanced applications and methodologies in biology and biotechnologies.

Metabolomics by Mass Spectrometry. Targeted and Untargeted metabolic profiling, Database Searching, Structural Identification by MS/MS Analysis. High-Definition Mass Spectrometry.

Proteomics and peptidomics by Mass Spectrometry. Targeted and Untargeted analysis. Bioinformatics software research. Comparison of several activation modes (CID, HCD, ETD). Product ions nomenclature and formation. Bottom up and top down analysis. Digestion protocols. Optimization of the chromatographic separation for high selective and sensible HRMS detection.

Course delivery

The course is delivered face-to-face, in class lectures and lab visits. The total course time is 32 hours.

The platform course will welcome: lesson materials; exercises to be carried out face-to-face; in-depth teaching materials that can be discussed through a forum and / or in the classroom during the lesson-debate. The objective of the learning environment is to create continuity between the different teaching moments and between the different situations (remote activities and face-to-face activities).

Learning assessment methods

Written evaluation of a scientific paper including the laboratory experiences by the use of a high resolution mass spectrometry analyzer

Support activities

Laboratory practical activity on a high resolution MS system

Suggested readings and bibliography (Mass Spectrometry Reviews, Journal of the American Society of Mass Spectrometry, Journal of Mass Spectrometry, Journal of Proteomics)

Mass Spectrometry Reviews

Journal of the American Society of Mass Spectrometry

Journal of Mass Spectrometry

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