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ADVANCED MASS SPECTROMETRY
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ADVANCED MASS SPECTROMETRY
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Academic year 2023/2024
- Course ID
- BIO0217 Pds GEN
- Teachers
- Federica Dal Bello
Claudio Medana - Degree course
- [0101M21] Molecular Biotechnology
- Year
- 2nd year
- Teaching period
- Second semester
- Type
- Elective
- Credits/Recognition
- 4
- Course disciplinary sector (SSD)
- CHIM/01 - analytical chemistry
- Delivery
- Formal authority
- Language
- English
- Attendance
- Obligatory
- Type of examination
- Written
- Type of learning unit
- corso
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Sommario del corso
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Course objectives
Acquisition of competences in the field of mass spectrometry analyses.
The objective of the course is to provide students with the principles for the development and application of methods based on (high resolution) mass spectrometry in the biochemical and biopharmaceutical fields.
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Results of learning outcomes
Analytical methods study and development skills
At the end of the course, students will be able to:
- Understand the value of mass spectrometry techniques to address biochemical and biotechnological problems
- Know when and how applying specific mass spectrometry techniques
- Analyze the obtained results (autonomy of judgment)
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Program
LC-MS and LC-HRMS applications. Advanced applications and methodologies in biology and biotechnologies.
Metabolomics by Mass Spectrometry. Targeted and Untargeted metabolic profiling, Database Searching, Structural Identification by MS/MS Analysis. High-Definition Mass Spectrometry.
Proteomics and peptidomics by Mass Spectrometry. Targeted and Untargeted analysis. Bioinformatics software research. Comparison of several activation modes (CID, HCD, ETD). Product ions nomenclature and formation. Bottom up and top down analysis. Digestion protocols. Optimization of the chromatographic separation for high selective and sensible HRMS detection.
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Course delivery
The course is delivered face-to-face, in class lectures and lab visits. The total course time is 32 hours.
The platform course will welcome: lesson materials; exercises to be carried out face-to-face; in-depth teaching materials that can be discussed through a forum and / or in the classroom during the lesson-debate. The objective of the learning environment is to create continuity between the different teaching moments and between the different situations (remote activities and face-to-face activities).
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Learning assessment methods
Written evaluation of a scientific paper including the laboratory experiences by the use of a high resolution mass spectrometry analyzer
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Support activities
Laboratory practical activity on a high resolution MS system
Suggested readings and bibliography (Mass Spectrometry Reviews, Journal of the American Society of Mass Spectrometry, Journal of Mass Spectrometry, Journal of Proteomics)
Suggested readings and bibliography
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Mass Spectrometry Reviews
Journal of the American Society of Mass Spectrometry
Journal of Mass Spectrometry
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