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Image and Signal Analysis I

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Analisi delle Immagini e dei Segnali I

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Academic year 2019/2020

Course ID
BIO0105
Teacher
Prof. Ferdinando Di Cunto
Degree course
[0101M21] Molecular Biotechnology
Year
2nd year
Type
Distinctive
Credits/Recognition
5
Course disciplinary sector (SSD)
BIO/11 - biologia molecolare
Delivery
Formal authority
Language
English
Attendance
Obligatory
Type of examination
Written and oral (optional)
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Sommario del corso

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Course objectives

The objective of this course will be to address the biological and technological problems of microscopy analysis, with particular emphasis on the analysis of living tissues and cells. The course will then analyze the techological features of the most advaced microscopy techniques, underscoring the interrelationships with the moste advanced image analysis techniques.

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Results of learning outcomes

Students are expected to acquire advanced theroetical understanding of advanced microscopy techniques, as well as a good knowledge of their application spectrum.

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Course delivery

Frontal lessons and guided seminars

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Learning assessment methods

The seminars and ad-hoc questioning will be used to monitor learning during the course.

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Support activities

Practical session with image-J image analysis software

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Program

- Basic concepts of microscopy.

- Problems of in vivo cell and tissue imaging: phototoxicity

- General concepts of advanced microscopy: pushing microscopy to its limits

- Confocal microscopy, conventional and spinning disk.

- TIRF microscopy.

- Light-sheet microscopy.

- Multiphoton microscopy.

- Analysis of gene expression via image analysis techniques.

- High content microscopy, analysis of mitosis.

- Tracking of cells and cell structures.

- Analysis of molecular dynamics through microscopy.

- 3D analysis of complex biological structures.

- Super-resolution microscopy.

- Optical manipulation of physiological systems: optogenetics

- Practical session with image-J image analysis software

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Suggested textbooks and readings

Optical Imaging and Microscopy

Techniques and Advanced Systems

Editors: Török, Peter, Kao, Fu-Jen (Springer)

 

https://www.sciencedirect.com/science/article/pii/S0891061810000839

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Last update: 04/10/2019 11:28
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