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Spectroscopy of biomolecules
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Spectroscopy of biomolecules
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Academic year 2020/2021
- Course ID
- BIO0153B Pds 307-DDD
- Teaching staff
- Prof.ssa Simonetta Geninatti Crich
Dott. Giuliana Magnacca (Lecturer) - Modular course
- SUPRAMOLECULAR CHEMISTRY AND SPECTROSCOPIC METHODS (BIO0153 Pds 307-DDD)
- Degree course
- [0101M21] Molecular Biotechnology
- Year
- 1st year
- Teaching period
- To be defined
- Type
- Distinctive
- Credits/Recognition
- 5
- Course disciplinary sector (SSD)
- CHIM/02 - chimica fisica
CHIM/03 - chimica generale e inorganica - Delivery
- Formal authority
- Language
- English
- Attendance
- Obligatory
- Type of examination
- Written and oral
- Prerequisites
- Basic knowledge in Inorganic Chemistry, Physical Chemistry, Biochemistry
- Propedeutic for
- All courses and practical activities dealing also with methods based on molecular spectroscopies
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Sommario del corso
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Course objectives
English:
The objectives proposed to students in this course are based on the following achievements:
1. knowledge of the chemical, physical and physical-chemical principles at the basis of the generation of signals exploited in molecular spectroscopies of biomolecules, namely NMR spectroscopy and electron spectroscopies (both in absorption and emission)
2. good capability in critical understanding scientific texts
3. good capability in designing investigation of different samples by molecular spectroscopies, on the basis on their constitutive features, possible constrains related to limited amounts, types of outputs requested (identification; quantification)
4. good capability in the complementary use of the molecular spectroscopic methods indicated above
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Results of learning outcomes
- Knowledge of main sample handling methods for NMR and electronic spectroscopic (both in absorption and emission) measurements
- Capability to design proper spectroscopic measurements for the investigation of biomolecular samples, taking into account the peculiar features of these samples (e.g, complexity of the matrix), and the specific target(s) to be pursued (identification/quantification)
- Capability to analyse data resulting from the methods indicated above.
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Course delivery
The course will be delivered through lessons in the lecture room (90%) and in labs (10%), for focus on some methodological aspects.
Part of the course will be devoted to NMR (3 CFU, 24 h) and part to electronic (absorption and emission) spectroscopy (2 CFU, 16 h)- Oggetto:
Learning assessment methods
The exam is devoted to the assessment of the knowledge, and related understanding, of the program attained by the students. In addition, also knowledge and understanding of basic knowledge in Chemistry and Biochemistry which should be necessary consider will be evaluated, as well as the use of a proper scientific/technical language.
The examination is carried out in oral form. The final mark is expressed in thirtieths.
Due to the COVID-19 sanitary emergency, the exams could be sustained via webex or alternative platforms.
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Program
Absorption electronic spectroscopy:
- origin of the energy transfer from the electromagnetic radiation to molecular electronic states
- types of electronic levels and electronic transitions
- solvatochromism
- UV circular dichroism
- study of the protein structure by absorption electronic spectroscopy (principles, experimental methods)
- study of the structure of nucleic acids by absorption electronic spectroscopy (principles, experimental methods)
Photoluminescence electronic spectroscopy:
- radiative and radiation less decays from excited electronic states
- fluorescence and phosphorescence
- radiative and fluorescence lifetimes
- collisional quenching
- energy transfer through the space
study of the protein structure by photoluminescence spectroscopy (principles, experimental methods)
- study of the structure of nucleic acids by photoluminescence spectroscopy (principles, experimental methods)
Nuclear Magnetic Resonance (NMR) Spectroscopy applications in the determination of structure, dynamics, and interactions of biological macromolecules:
- Introduction to the NMR spectral parameters used in structural biology, namely the chemical shift, the J-coupling, nuclear Overhauser effects, and residual dipolar couplings.
- Resonance assignment, and NMR spectral parameters are their convertion into angle and distances between atoms
in a macromolecules.
-Relaxation phenomena and mapping the molecular interactions, information on the binding interface as well as the determination of kinetic and thermodynamic constants..
-NMR studies on Metalloproteins (proteins containing at least one metal ion) and paramagnetism based drug discovery.
- Nucleic acids studied by NMR and their interaction with metallo drugs.
- In cell/In tissue NMR spectroscopy.
Suggested readings and bibliography
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Reference material is available at the course website.
Suggested textbooks (all available from the teachers):
- H. Friebolin “Basic one- and two-dimensional NMR spectroscopy”, VCH, 1993
- Ivano Bertini, Kathleen S. McGreevy, Giacomo Parigi “NMR of biomolecules” Wiley 2012.
- DW. Claridge “High-resolution NMR techniques in Organic Chemistry”, Pergamon 1999 (Elsevier Science)
N.J. Turro, Modern Molecular Photochemistry, University Science Books, 1991
C.N.R. Rao, Ultra-Violet and Visible Spectroscopy, Butterworths, third edition, 1966
J.R. Lakowitz, Principles of fluorescence spectroscopy, Plenum, 2000 I.D. Campbell
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Note
The course will be delivered online with live lessons provided at the Moodle elearning platform at the following link
https://biotec.i-learn.unito.it/
Due to the COVID-19 sanitary emergency, the modality of course delivery could vary during the year. In any case, the "online" teaching will be guaranteed for the entire academic year.
Il corso sarà erogato in modalità telematica con lezioni in sincrono fruibili connettendosi alla piattaforma Moodle elearning al seguente link
https://biotec.i-learn.unito.it/
Le modalità di svolgimento dell'attività didattica potranno subire variazioni in base alle limitazioni imposte dalla crisi sanitaria in corso. In ogni caso è assicurata la modalità a distanza per tutto l'anno accademico
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