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Nanomaterials
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Nanomaterials
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Academic year 2020/2021
- Course ID
- BIO0168B Pds 306-TB
- Teaching staff
- Prof. Gabriele Ricchiardi
Dott. Giuliana Magnacca - Modular course
- CHEMISTRY FOR HEALTH SCIENCES (BIO0168 Pds 306-TB)
- Degree course
- [0101M21] Molecular Biotechnology
- Year
- 2nd year
- Teaching period
- To be defined
- Type
- Distinctive
- Credits/Recognition
- 5
- Course disciplinary sector (SSD)
- CHIM/02 - chimica fisica
- Delivery
- Formal authority
- Language
- English
- Attendance
- Obligatory
- Type of examination
- Oral
- Prerequisites
- - basic knowledge in Physical-Chemistry (structure of matter; intermolecular interactions; thermodynamic of molecular systems; main molecular spectroscopic methods)
- basic knowledge in Physiscs (nautire of electromagnetic radiation, electric dipoles; coulomb interactions; elastic and inelastic interactions)
- basic knowledge in biochemistry - Course borrowed from
- Nanomaterials (BIO0160B Pds 307-DDD)Master Program in Molecular Biotechnology
- Nanomaterials (BIO0160B Pds 307-DDD)
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Sommario del corso
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Course objectives
The objectives proosed to the studentd as targets of this course are aimed to the achievements of good capabilities dealing with:
- establishment of relationships between the features of amphiphlic molecules/self-assembled amphiphilic molecules and their functional behaviours relevant for their use in nanobiotechnology
- establishment of relationships between features of inorganic nanoparticles and their functional behaviours relevant for their use in nanobiotechnology
- selection and aplication of proper experimental methods for the study of nanobiotecnological systems- Oggetto:
Results of learning outcomes
Knowledge of and expertise in:
- main methods for the preparation of nanomaterials of interest in the
field of biotechnology
- fundamental aspects of physical and physical-chemical features of nanomaterials at the basis of their possible exploitation in nanobiotechnology
- fundamental aspects of the combined use of nanoparticles and biomacromolecules
- experimental methods for the characterization of nanobiotechnologycal systems, and selection of the most proper ones in dependence on specific features of the systems
- electron microscopy, and capability to design the proper observation methods for the investigation of samples of biological/biotechnological interest.- Oggetto:
Course delivery
- Traditional + visit at the EM lab
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Learning assessment methods
For the autumn sessions 2020, the examination will be in oral form via Webex (one of the possible choiches present in the Rectoral Decree 2576/2020 on 23.07.2020).This info will be updated when the University will communicate new rules.In general, in a future, when exams in presence will be the only form allowed: written examination with 5 open questions.- Oggetto:
Program
This course is devoted to the knowledge and understanding of physical-chemical features of naomaterials at the basis of their functional behaviours which can be exploited for biotechnological applications.
The topics will then deal with:
"soft" nanomaterials
- self-assembling of amphiphilic molecules resulting in the formation of micelles
- relationships between compositional and structural features of amphiphilic molecules and structure and functional behaviour of micelles
- methods for the characterization of micelles-based nanomaterials
- cases-study related to actual or potential application of self-assembled soft materials in biotechnology (e.g, drug delivery, gene therapy)"hard" nanomaterials
- oxide nanoparticles (typically made of silica), hybridized with fluorophores, intented for applications in "in vitro" and "in vivo" optical imaging: preparation, characterization, surface functionalization for targeting, possible uses
- metal nanoparticles(typically made of gold): origin and dependences of peculiar optical behaviours (plasmon resonance, effect on the emission features of nearby fluorophores), possible uses in cellular and molecular biology
TImaging of nanoparticles: electron microscopy
Scanning electron microscopy: principles; signals to be exploited for the formation of images; type of information present in the images depending on teh signal collected; sample preparation; uses in biology/biotechnology
Transmission electron microscopy: principles; origin and importance of the high resolution, sample preparation; uses in biology/biotechnology
Chemical analysis by energy dispersion spectroscopy of X-rays emitted by samples impinged by a beam of accelerated electrons: principles; uses in biology/biotechnologySuggested readings and bibliography
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- I.A. Israelechvili, Intermolecular and surface forces, Wiley&Sons, Chichester-UK, 1999, second edition
- J.I. Goldstein et al., Scanning Electron Microscopy and X-Ray Microanalysis : A Text for Biologists, Materials Scientists, and Geologists, Springer-Verlag New York Inc., New York 2011,
- William & Carter, Transmission electron Microscopy, Interscientia, San Diego, 2002, seconda edizione
- scientific papers, uploaded on the webpage of the course
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Note
Le modalità di svolgimento dell'attività didattica potranno subire variazioni in base alle limitazioni imposte dalla crisi sanitaria in corso. In ogni caso è assicurata la modalità a distanza per tutto l'anno accademico
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