IN VITRO DIAGNOSTICS: MOLECULAR PROFILING OF PROLIFERATIVE PROCESSES
In vitro diagnostics: molecular profiling of proliferative processes
Academic year 2022/2023
- Course ID
- BIO0171 Pds 306-TB
- Prof. Roberto Piva
Prof.ssa Claudia Voena
- Degree course
- [0101M22] Molecular Biotechnology
- 1st year
- Teaching period
- Second semester
- Course disciplinary sector (SSD)
- MED/08 - pathology
- Formal authority
- Type of examination
Sommario del corso
The course aims to show how emerging technologies have contributed and still contribute to the comprehension of cancer and have changed the diagnostic procedures and therapeutic options for cancer patients, mainly focusing on hematological tumors. Through frontal lessons and the discussion of recent literature and milestones in this field of research, the course will provide the students with an overview of the impact of technology advancement on cancer research and with critical perspectives upon current and emerging cancer therapies, how they are developed, and how they are applied in the clinical setting. In addition, the most recent methodologies applied to cancer research will be discussed in detail.
Results of learning outcomes
Students will gain knowledge of the progress in the diagnostic and treatment of cancer due to the advancement of biotechnology in this research field.
Students will consolidate and develop a broad understanding of fundamental bases and emergent areas in the field of cancer, from the principles and applications of key techniques in molecular oncology to the identification of cancer vulnerabilities for targeted treatment.
The presentation sessions will allow students to develop critical reading, improve presentation skills, and critically assess experimental approaches and methodologies applied to cancer research.
The peer-review process
DNA Sequencing: Whole Exome Sequencing, Whole Genome Sequencing, Target sequencing
Expression profiling: from microarrays to (single-cell) RNA sequencing
The Cancer Genome Atlas (TCGA) – The Pan-Cancer Analysis Project - The Pan-Cancer Analysis of Whole Genomes (PCAWG) Consortium
Clonal Hematopoiesis and The Pre-Cancer Genome Atlas
Epigenetics and cancer
Hematological tumors: how molecular biology has revolutionized hematological tumor classification and therapy
Functional genomics: RNA interference, CRISPR/CAS9, drug screening
Tumor heterogeneity and clonal evolution
Cancer vulnerabilities, drug resistance, persistence, and quiescence
Proteomics, proteogenomics for cancer diagnosis and treatment
The course is held 3 hours a week, for a total of 40 hours.
Each lesson is divided into 2 sessions:
- a frontal lesson with a slide presentation given by the teacher;
- students' presentations of scientific papers including either a critical review or a methodological description of key and emerging technologies. All students are strongly encouraged to actively participate in the discussion.
Learning assessment methods
The presentation in class and other additional assignments such as paper peer-reviewing and gene variant classification will be evaluated as 1/3 of the total vote.
The final oral examination will evaluate the knowledge of the subjects of the course, the critical thinking skills, and the quality of the exposition. The vote will be expressed as a fraction of 30 (eventually cum laude).
Teachers are available for students by email or upon appointment to discuss issues related to the course.
Suggested readings and bibliography
- The biology of cancer weinberg - 2nd edition
- Year of publication:
- Garland Science
- Robert A. Weinberg
Slides and selected papers will be provided during the course.
Le modalità di svolgimento dell'attività didattica potranno subire variazioni in base alle limitazioni imposte dalla crisi sanitaria in corso. In ogni caso è assicurata la modalità a distanza per tutto l'anno accademico