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IN VITRO DIAGNOSTICS: PROTEOMICS, INTERACTOMICS AND METABOLOMICS

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IN VITRO DIAGNOSTICS: PROTEOMICS, INTERACTOMICS AND METABOLOMICS

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Academic year 2023/2024

Course ID
BIO0162 Pds 308-BIDD
Teachers
Paola Defilippi
Paolo Ettore Porporato
Alessandra Ghigo
Degree course
[0101M22] Molecular Biotechnology
Year
2nd year
Teaching period
Second semester
Type
Distinctive
Credits/Recognition
5
Course disciplinary sector (SSD)
BIO/13 - experimental biology
Delivery
Formal authority
Language
English
Attendance
Obligatory
Type of examination
Oral
Type of learning unit
corso
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Sommario del corso

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Course objectives

The aim of the course is to provide the students with an overview of the principal -omics techniques as well as their applications in basic and translational research studies in the field of biomedicine.

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Results of learning outcomes

Knowledge of the -omics techniques most commonly used in biomedical research.

Ability to apply the knowledge of the different -omics techniques to specific biological problems.

Critical analysis of -omics-based research studies.

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Program

1) Interactomics

Overview of most commonly used tehcniques for studying protein-protein interaction, pros and cons of each technique, practical examples. Lysis-dependent methods: surface plasmon resonance, pull down, far western blot, co-immunoprecipitation. Lysis-independent approaches: proximity ligan assay, proximity-dependent labeling approaches such as BioID, TurboID, APEX, VitoTrap.

2) Metabolomic

Overview of various techniques useful to study  metabolic dysregulation. Analysis of the recent evolution in many paradigm shift in the field of cancer metabolism and analysis of main recent paper relevant in the metabolism field to appreciate the technical evolution of the recent yers in the field

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Course delivery

Formal lessons and seminars, with a high degree of interactivity to stimulate discussion of the topics presented.

 

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Learning assessment methods

Oral examination including the presentation and critical discussion of a scientific publication. Evaluation will be based on quality of the presentation, knowledge of the subjects of the course and critical thinking skills. The vote will be expressed as a fraction of 30 (eventually cum laude).

Suggested readings and bibliography



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