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IN VITRO DIAGNOSTICS: PROTEOMICS, INTERACTOMICS AND METABOLOMICS
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IN VITRO DIAGNOSTICS: PROTEOMICS, INTERACTOMICS AND METABOLOMICS
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Academic year 2024/2025
- Course ID
- BIO0162 Pds 308-BIDD
- Teachers
- Paola Defilippi
Paolo Ettore Porporato
Alessandra Ghigo - Degree course
- [0101M22] Molecular Biotechnology
- Year
- 2nd year
- Teaching period
- Second semester
- Type
- Distinctive
- Credits/Recognition
- 5
- Course disciplinary sector (SSD)
- BIO/13 - experimental biology
- Delivery
- Formal authority
- Language
- English
- Attendance
- Obligatory
- Type of examination
- Oral
- Type of learning unit
- corso
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Sommario del corso
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Course objectives
The aim of the course is to provide the students with an overview of the principal -omics techniques as well as their applications in basic and translational research studies in the field of biomedicine.
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Results of learning outcomes
Knowledge of the -omics techniques most commonly used in biomedical research.
Ability to apply the knowledge of the different -omics techniques to specific biological problems.
Critical analysis of -omics-based research studies.
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Program
1) Interactomics
Overview of most commonly used tehcniques for studying protein-protein interaction, pros and cons of each technique, practical examples. Lysis-dependent methods: surface plasmon resonance, pull down, far western blot, co-immunoprecipitation. Lysis-independent approaches: proximity ligan assay, proximity-dependent labeling approaches such as BioID, TurboID, APEX, VitoTrap.
2) Metabolomic
Overview of various techniques useful to study metabolic dysregulation. Analysis of the recent evolution in many paradigm shift in the field of cancer metabolism and analysis of main recent paper relevant in the metabolism field to appreciate the technical evolution of the recent yers in the field
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Course delivery
Formal lessons and seminars, with a high degree of interactivity to stimulate discussion of the topics presented.
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Learning assessment methods
Oral examination including the presentation and critical discussion of a scientific publication. Evaluation will be based on quality of the presentation, knowledge of the subjects of the course and critical thinking skills. The vote will be expressed as a fraction of 30 (eventually cum laude).
Suggested readings and bibliography
- Enroll
- Open
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