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Nanomaterials

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Nanomaterials

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Academic year 2021/2022

Course ID
BIO0168B Pds 306-TB
Teachers
Prof. Gabriele Ricchiardi
Prof.ssa Giuliana Magnacca
Degree course
[0101M21] Molecular Biotechnology
Year
2nd year
Teaching period
To be defined
Type
Distinctive
Credits/Recognition
5
Course disciplinary sector (SSD)
CHIM/02 - chimica fisica
Delivery
Formal authority
Language
English
Attendance
Obligatory
Type of examination
Oral
Type of learning unit
modulo
Modular course
CHEMISTRY FOR HEALTH SCIENCES (BIO0168 Pds 306-TB)
Prerequisites
- basic knowledge in Physical-Chemistry (structure of matter; intermolecular interactions; thermodynamic of molecular systems; main molecular spectroscopic methods)
- basic knowledge in Physiscs (nautire of electromagnetic radiation, electric dipoles; coulomb interactions; elastic and inelastic interactions)
- basic knowledge in biochemistry
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Sommario del corso

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Course objectives

The objectives proosed to the studentd as targets of this course are aimed to the achievements of good capabilities dealing with:
- establishment of relationships between the features of amphiphlic molecules/self-assembled amphiphilic molecules and their functional behaviours relevant for their use in nanobiotechnology
- establishment of relationships between features of inorganic nanoparticles and their functional behaviours relevant for their use in nanobiotechnology
- selection and aplication of proper experimental methods for the study of nanobiotecnological systems

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Results of learning outcomes

Knowledge of and expertise in:
- main methods for the preparation of nanomaterials of interest in the
field of biotechnology
- fundamental aspects of physical and physical-chemical features of nanomaterials at the basis of their possible exploitation in nanobiotechnology
- fundamental aspects of the combined use of nanoparticles and biomacromolecules
- experimental methods for the characterization of nanobiotechnologycal systems, and selection of the most proper ones in dependence on specific features of the systems
- electron microscopy, and capability to design the proper observation methods for the investigation of samples of biological/biotechnological interest.

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Program

This course is devoted to the knowledge and understanding of physical-chemical features of naomaterials at the basis of their functional behaviours which can be exploited for biotechnological applications.
The topics will then deal with:
"soft" nanomaterials
- self-assembling of amphiphilic molecules resulting in the formation of micelles
- relationships between compositional and structural features of amphiphilic molecules and structure and functional behaviour of micelles
- methods for the characterization of micelles-based nanomaterials
- cases-study related to actual or potential application of self-assembled soft materials in biotechnology (e.g, drug delivery, gene therapy)

"hard" nanomaterials
- oxide nanoparticles (typically made of silica), hybridized with fluorophores, intented for applications in "in vitro" and "in vivo" optical imaging: preparation, characterization, surface functionalization for targeting, possible uses
- metal nanoparticles(typically made of gold): origin and dependences of peculiar optical behaviours (plasmon resonance, effect on the emission features of nearby fluorophores), possible uses in cellular and molecular biology
TImaging of nanoparticles: electron microscopy
Scanning electron microscopy: principles; signals to be exploited for the formation of images; type of information present in the images depending on teh signal collected; sample preparation; uses in biology/biotechnology
Transmission electron microscopy: principles; origin and importance of the high resolution, sample preparation; uses in biology/biotechnology
Chemical analysis by energy dispersion spectroscopy of X-rays emitted by samples impinged by a beam of accelerated electrons: principles; uses in biology/biotechnology

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Course delivery

  1. Traditional + exercises on electron microscopy images
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Learning assessment methods

The exams will be in oral form via Webex (Gabriele Ricchiardi's webex room) unless new rules will be communicated.
 
In general, in a future, when exams in presence will be the only form allowed: written examination with 5 open questions.

Suggested readings and bibliography

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- I.A. Israelechvili, Intermolecular and surface forces, Wiley&Sons, Chichester-UK, 1999, second edition

- J.I. Goldstein et al., Scanning Electron Microscopy and X-Ray Microanalysis : A Text for Biologists, Materials Scientists, and Geologists, Springer-Verlag New York Inc., New York 2011, 

- William & Carter, Transmission electron Microscopy, Interscientia, San Diego, 2002, seconda edizione

- scientific papers, uploaded on the webpage of the course



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Notes

Teaching will occur in the mixed form: in presence and via webex streaming.

The first 24 hours of the course will be held by Gabriele Ricchiardi (webex link: https://unito.webex.com/meet/gabriele.ricchiardi), the second part of the course will be held by Giuliana Magnacca (webex link: https://unito.webex.com/meet/giuliana.magnacca).

Some modifications could occur: in case, they will be communicated via email.

 

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