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Nanomaterials
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Nanomaterials
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Academic year 2024/2025
- Course ID
- BIO0160B Pds 308-BIDD
- Teachers
- Gabriele Ricchiardi (Lecturer)
Giuliana Magnacca - Degree course
- [0101M22] Molecular Biotechnology
- Year
- 2nd year
- Teaching period
- First semester
- Type
- Distinctive
- Credits/Recognition
- 5
- Course disciplinary sector (SSD)
- CHIM/02 - physical chemistry
- Delivery
- Formal authority
- Language
- English
- Attendance
- Obligatory
- Type of examination
- Oral
- Type of learning unit
- modulo
- Modular course
- ADVANCED MATERIALS AND METHODS FOR HEALTH SCIENCES (BIO0160 Pds 308-BIDD)
- Prerequisites
- - basic knowledge in Physical-Chemistry (structure of matter; intermolecular interactions; thermodynamic of molecular systems; main molecular spectroscopic methods)
- basic knowledge in Physics (nature of electromagnetic radiation, electric dipoles; Coulomb interactions; elastic and inelastic interactions)
- basic knowledge in biochemistry - Oggetto:
Sommario del corso
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Course objectives
The objectives proosed to the studentd as targets of this course are aimed to the achievements of good capabilities dealing with:
- establishment of relationships between the features of amphiphlic molecules/self-assembled amphiphilic molecules and their functional behaviours relevant for their use in nanobiotechnology
- establishment of relationships between features of inorganic nanoparticles and their functional behaviours relevant for their use in nanobiotechnology
- selection and aplication of proper experimental methods for the study of nanobiotecnological systems- Oggetto:
Results of learning outcomes
A: KNOWLEDGE AND UNDERSTANDING
- main methods for the preparation and characterization of main classes of nanomaterials of interest in biotechnology
- fundamental aspects of physical and physical-chemical features of nanomaterials at the basis of their possible exploitation in biotechnology
- fundamental aspects of the combined use of nanoparticles and biomacromolecules
- electron microscopy of biological/biotechnological interest.
B: APPLYING KNOWLEDGE AND UNDERSTANDING
- establishing correct causal relationships when discussing the fnctional behaviour of nanomaterials
- selection of the effective characterization techniques of nanomaterials of interest for biotechnology
- capability to select the proper electron microscopy methods and signals for the investigation of samples
C: MAKING JUDGEMENTS
- capability to critically analysing the scientific literature
D: COMMUNICATION
- abilty to use the technical vocabulary of research in the field
E: LIFELONG LEARNING SKILLS - ability to understand future literature in the field of nanomaterials and electron microscopy, in particular when applied to biotechnology
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Program
This course is devoted to the knowledge and understanding of physical-chemical features of naomaterials at the basis of their functional behaviours which can be exploited for biotechnological applications.
The topics will then deal with:
"soft" nanomaterials
- self-assembling of amphiphilic molecules resulting in the formation of micelles
- relationships between compositional and structural features of amphiphilic molecules and structure and functional behaviour of micelles
- methods for the characterization of micelles-based nanomaterials
- cases-study related to actual or potential application of self-assembled soft materials in biotechnology (e.g, drug delivery, gene therapy)"hard" nanomaterials
- oxide nanoparticles (typically made of silica), hybridized with fluorophores, intented for applications in "in vitro" and "in vivo" optical imaging: preparation, characterization, surface functionalization for targeting, possible uses
- metal nanoparticles(typically made of gold): origin and dependences of peculiar optical behaviours (plasmon resonance, effect on the emission features of nearby fluorophores), possible uses in cellular and molecular biology
Imaging of nanoparticles: electron microscopy
Scanning electron microscopy: principles; signals to be exploited for the formation of images; type of information present in the images depending on teh signal collected; sample preparation; uses in biology/biotechnology
Transmission electron microscopy: principles; origin and importance of the high resolution, sample preparation; uses in biology/biotechnology
Chemical analysis by energy dispersion spectroscopy of X-rays emitted by samples impinged by a beam of accelerated electrons: principles; uses in biology/biotechnology- Oggetto:
Course delivery
The topic "soft and hard nanomaterials" (3CFU) will be taught by Prof. Ricchiardi, while the topic "electron microscopy" (2CFU) will be taught by Prof. Martra.
Traditional lessons in presence.
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Learning assessment methods
Oral examination (approx 30-4o min).Suggested readings and bibliography
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- I.A. Israelechvili, Intermolecular and surface forces, Wiley&Sons, Chichester-UK, 1999, second edition
- J.I. Goldstein et al., Scanning Electron Microscopy and X-Ray Microanalysis : A Text for Biologists, Materials Scientists, and Geologists, Springer-Verlag New York Inc., New York 2011,
- William & Carter, Transmission electron Microscopy, Interscientia, San Diego, 2002, second edition
- scientific papers, uploaded on the webpage of the course
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Courses that borrow this teaching
- Nanomaterials (BIO0168B Pds 306-TB)Master Program in Molecular Biotechnology
- Nanomaterials (BIO0168B Pds 306-TB)
- Enroll
- Open
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